White Papers and Tech Notes

Life Science Webinars _ Corning

Watch Seminar Presentation

Watch BennuBio President and CEO, John O'Rourke, give a talk on a simplified approach for rapid analysis of thousands of intact spheroids using the Velocyt® LP Large Particle Flow Cytometer as part of a collaboration with Corning.

Watch Video

AppNote

Ditch The Dissociation: A Simplified Approach for Rapid High Throughput Multiplexed Analysis of Intact 3D Multicellular Spheroids using the Velocyt Cytometer

BennuBio White Paper

In this application note, we demonstrate the use of the Velocyt® cytometer for the spheroid workflow using staurosporine treatment as a model drug compound. In this case study, we illustrate Bennubio’s simplified spheroid solution approach (S³) to generate and treat large numbers of uniform spheroids, a simplified sample preparation workflow and the rapid analysis of thousands of intact spheroids, generating flow cytometry, fluorescence imaging and morphological data from each spheroid. 

Download the full App Note

Poster

Streamlining 3D Model Analysis: High-Throughput Applications of the Velocyt Cytometer in Suspension and Static Cultures

In the first case study, we assessed the size distribution and scatter parameters of iPSC grown in suspension cultures. Next, we measured apoptosis from pancreatic spheroids grown in microwell dishes after treatment with staurosporine. Finally, we used colon cancer spheroids to analyze immune cell penetrance and cancer cell death in a co-culture setting.

Download the poster

CORG

Enhancing 3D Culture Analysis: Media Optimization and Immune Cell Killing Assays Using the Velocyt Cytometer

In this study, we present two case studies that highlight the versatility and efficiency of the Velocyt. The first involves a longitudinal assessment of size distribution and viability of 3D cultures grown in stirred bioreactors under different media formulations. The second utilizes microwell technology to perform immune cell killing assays, measuring both immune cell penetration and 3D culture viability.

These examples underscore the Velocyt’s ability to simplify workflows and its value in diverse 3D applications, offering a robust solution for the challenges inherent in high-throughput 3D model analysis.

Download the poster